In fact, the solvent polarity used and the solubility determined the nature of the compounds present in each plant extract (Mahmoudi et al. Chabi, N. W., C. T. R. Konfo, M. Adjagba, L. Moussedikou, E. Ahoussi-Dahouenon, A. Laleye, C. Gbaguidi, and M. M. Soumanou. With the three extracts, the seeds did not lose their viability. Progeny production of Callosobruchus maculatus in grains treated with three extracts of Hemizygyia welwitschii leaf powder under ambient laboratory conditions. Executive summary report, Interception of insect pests in exotic seed germplasm, Spinosad: a new natural product for stored grain protection, Bioactivity of novel botanical insecticide from, Activation of octopaminergic receptors by essential oil constituents isolated from aromatic plants: possible mode of action against insect pests, Etude de l’extraction des composés phénoliques de différentes parties de la fleur d’artichaut (, A multivariate analysis of morphological variation in the, Insecticidal activity of the root extract of, Manuel de manipulation des semences dans les banques de gènes. Unfortunately, the grain is extensively infested by a number of insect pests including Callosobruchus maculatus, which is a primary field to store pest causing considerably great losses to farmers (Ojo et al. For 3, 5, and 7 d after exposure, hexane extract was more toxic to the bruchids. Search for other works by this author on: Department of Biological Sciences, University of Bamenda, Bamenda, Cameroon, Center for Research on Medicinal Plants and Traditional Medicine (CRPMT), Institute of Medical Research and Medicinal Plants Studies, Yaoundé, Cameroon, Julius Kühn-Institut, Institute for Ecological Chemistry, Plant Analysis and Stored Products Protection, Königin-Luise Str.19, Berlin, Germany, A method of computing the effectiveness of an insecticide, Preliminary phytochemical analysis and insecticidal activity of ethanolic extracts of four tropical plants (, Association Française de Normalisation (AFNOR), Recueil des normes françaises des produits dérivés des fruits et légumes. With the perspective of providing quality food for population, the interest of researchers has been focused to find alternatives to synthetic chemicals which could be environmentally friendly and do not pose hazards to man (Alice and Srikanth 2013). Phytochemical investigation of the extracts from the plant used in the present study showed that it is rich in many active compounds including phenolic compounds, alkaloids, saponins, tannins, triterpenes, flavonoids, and sterols groups. Corrected cumulative mortality of Callosobruchus maculatus exposure to hexane, acetone and methanol leaf extracts of Hemizygia welwitschii. The insect census taken at one, two and three months showed a progressive increase in insect population from one month to three months. 0000007383 00000 n 0000002581 00000 n © The Author(s) 2019. Despite local availability of H. welwitschii, this plant is not yet used by farmers in pest control. Broken seeds and particles were removed and then disinfested by keeping them in a freezer at −18°C for 30 d prior to bioassays. Acetone extract within 1 d acted more rapidly (slope = 4.55 ± 0.74) than methanol extract (slope = 1.18 ± 0.21) and hexane extract (slope = 1.08 ± 0.42) and 5 d of exposure (slope = 1.25 ± 0.13). Fotso, T. G., E. N. Nukenine, R. Tchameni, J. W. Goudoungou, D. Kosini, V. Tigamba and C. Adler. 0000005710 00000 n 0000002420 00000 n The dried leaves powder of H. welwitschii (2.3 kg) was mixed with 10 liters of hexane (apolar solvent) in the perforated container and agitated for 30 min and allowed to stand for 24 h in the Phytochemistry Laboratory of Institute of Medical Research and Medicinal Plants Studies, Yaoundé, Cameroon, and then restirred again.